Table 3.
Mitochondrial respiratory efficiency.
| Control | HF | HF+KO | ||
|---|---|---|---|---|
| Palmitoyl-L-carnitine + malate (β oxidation) |
V 3 (nmol O2·mL−1·min−1) | 85.8 ± 7.8 | 59.7 ± 8.5* | 114.0 ± 11.2∗# |
| V 4 (nmol O2·mL−1·min−1) | 14.3 ± 2.4 | 22.9 ± 1.5* | 18.5 ± 5.2∗# | |
| RCR | 6.0 ± 0.4 | 2.6 ± 0.3* | 6.1 ± 0.8# | |
|
| ||||
| Pyruvate + malate (Complex I) |
V 3 (nmol O2·mL−1·min−1) | 17.0 ± 0.3 | 15.9 ± 0.4* | 19.9 ± 0.9∗# |
| V 4 (nmol O2·mL−1·min−1) | 5.4 ± 0.1 | 7.6 ± 0.2* | 6.6 ± 0.6∗# | |
| RCR | 3.2 ± 0.3 | 2.1 ± 0.3* | 3.0 ± 0.4# | |
|
| ||||
| Succinate + rotenone (Complex II) |
V 3 (nmol O2·mL−1·min−1) | 61.5 ± 3.3 | 53.1 ± 3.7* | 78.3 ± 4.0∗# |
| V 4 (nmol O2·mL−1·min−1) | 10.1 ± 0.3 | 25.0 ± 1.2* | 15.0 ± 1.9∗# | |
| RCR | 6.1 ± 0.9 | 2.1 ± 0.8* | 5.2 ± 1.0# | |
|
| ||||
| Ascorbate + TMPD + rotenone + antimycin A (Complex IV) |
V 3 (nmol O2·mL−1·min−1) | 55.1 ± 5.3 | 47.5 ± 6.5* | 68.6 ± 7.8∗# |
| V 4 (nmol O2·mL−1·min−1) | 9.3 ± 0.7 | 17.2 ± 0.9* | 12.8 ± 0.8∗# | |
| RCR | 5.9 ± 0.6 | 2.8 ± 0.3* | 5.4 ± 0.9# | |
Respiratory control ratio (RCR) was calculated as the ratio of the rate of oxygen uptake in the presence of added ADP (V 3) to the rate observed when added ADP had been completely phosphorylated to ATP (V 4).
* P < 0.05 versus rats fed control diet; # P < 0.05 versus rats fed HF diet.