Table 1.
qRT-PCR primer sequences for proinflammatory and anti-inflammatory cytokines.
| Gene | Forward (5′→3′) | Reverse (5′→3′) |
|---|---|---|
| Proinflammatory cytokines | ||
| IL-1 | CGCCAATGACTCAGAGGAAG | AGGGCGTCATTCAGGATCAA |
| IL-6 | GTAGCCGCCCCACACAGACAGCC | GCCATCTTTGGAAGGTTC |
| IL-8 | GAACTGAGAGTGATTGAGAGT | CTTCTCCACAACCCTCTG |
| IL-12 | TGGAGTGCCAGGAGGACAGT | TCTTGGGTGGGTCAGGTTTG |
| IL-17 | TGAAGGCAGGAATCACAAT | GGTGGATCGGTTGTAGTAAT |
| CCL8 | CACAAGAATCACCAACATCC | TGGTCCAGATGCTTCATG |
| CXCL10 | TCTGACTCTAAGTGGCATTC | ATTGTAGCAATGATCTCAACAC |
| G-CSF | AGCTTCCTGCTCAAGTGC | TTCTTCCATCTGCTGCCAGATGGT |
| ICAM-1 | CACAGTCACCTATGGCAA | CTGGCTTCGTCAGAATCA |
| IFN-γ | TCAGCTCTGCATCGTTTTGG | GTTCCATTATCCGCTACATCTGAA |
| LIF | CCTGGACAAGCTATGTGG | GGTTGAGGATCTTCTGGTC |
| TNF-α | TCTTCTCGAACCCCGAGTGA | CCTCTGATGGCACCACCAG |
|
| ||
| Anti-inflammatory cytokines | ||
| IL-1RA | GTTCCATTCAGAGACGATCT | GTTGTTCCTCAGATAGAAGGT |
| IL-10 | GTGATGCCCCAAGCTGAGA | CACGGCCTTGCTCTTGTTTT |
| IL-11 | GGACCACAACCTGGATTC | GCAGGTAGGACAGTAGGT |
| IL-13 | ATTGCTCTCACTTGCCTT | GTCAGGTTGATGCTCCAT |
| IL-18BP | ACCATGAGACACAACTGG | ATGCTGGACACTGCTTAG |
| IL-35 | CACGTCCTTCATCCTCAG | GACTCCAGTCACTCAGTTC |
| CCL2 | AGTCACCTGCTGTTATAACTT | CACAATGGTCTTGAAGATCAC |
| COX2 | ACAGTCCACCAACTTACAAT | CAATCATCAGGCACAGGA |
| HGF | TTATCCTGACGTAAACACCTTTGATATAAC | CTGGGCAGTATTCGGGTTTGA |
| PTGES2 | CCTGGAAGAGATCATCACC | CCTTCTCGTTGAGCATGA |
| STC-1 | AGGATGATTGCTGAGGTG | TGTTATAGTATCTGTTGGAGAAGT |
| TGF-β | CAGCAACAATTCCTGGCGATA | AAGGCGAAAGCCCTCAATTT |
| TNFRSF1A | CAGGAAGAACCAGTACCG | TTCTTACAGTTACTACAGGAGAC |
| TSG-6 | CATCTCGCAACTTACAAGC | AGACGGATTCCATAATCAATAATG |