Figure 2.

MSCs recruited to injured tissue and fused with hepatocytes. (a) The liver specimens were collected on days 14 and 21 after injury. FISH was used to detect Y-chromosome-positive cells, and IF was used to detect GFP-positive cells. The nuclei were stained blue (DAPI). The images were then merged. Bar: 75 μm. Arrow was directed to the double positive cells. All the experiments were repeated three times, at least 3 samples were included in each group. (b) Quantitative data of the GFP and Y-chromosome-positive cells in IHC of different days after injuring (^*** P < 0.001).