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. Author manuscript; available in PMC: 2016 Aug 15.
Published in final edited form as: Cancer Res. 2015 Jun 22;75(16):3216ā€“3226. doi: 10.1158/0008-5472.CAN-15-0584

Figure 4. Prospective characterization of effector CD8+ T cell clones based upon by IL-7R and c-myc expression.

Figure 4

(A) Phenotype of individual MART specific CD8+ T effector clones (n=7) isolated by limiting dilution from the peripheral blood of a metastatic melanoma patient. Numbers in FACS plot represent % CD8+Tetramer+ cells (upper panel) and % CD45RO+CD62Lāˆ’ cells (lower panel) compared to isotype. (B) Telomere length of isolated effector clones. (C) Cell surface IL-7R expression (MFI) and c-myc mRNA (per 105 b-actin copies) expression in isolated effector clones. (D) Cytokine induced pSTAT5 expression (% of cells) in isolated CD8+ effector clones (n=7). Numbers in histograms represent % of cells induced to express pSTAT5 by respective cytokine compared to no cytokine baseline.