Skip to main content
. Author manuscript; available in PMC: 2016 Sep 1.
Published in final edited form as: Microvasc Res. 2015 Jun 27;101:72–81. doi: 10.1016/j.mvr.2015.06.006

Figure 2. Human platelet lysate (HPL) in ECFC co-culture stimulates protein expression of apoptotic/ cell survival related molecules.

Figure 2

Figure 2

(a) Representative immunoblots of pro-survival (Akt1, pAkt1, pBad and Bcl-xL) and apoptotic (cleaved caspase-3) proteins from ECFC and ECFC with HPL in 3D matrices. Lower panels show quantification of repeated experiments on day 1 (b) and day 3(c) after culture (protein expression levels were normalized using β-actin). Pro-survival molecules and apoptotic molecule were significantly upregulated on co-culture of ECFC with HPL compared to ECFC alone on day 1 and day 3. N=2 each group ns=no significance, *P < 0.05 or **P<0.001.