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. 2015 Feb 16;34(43):5447–5459. doi: 10.1038/onc.2014.473

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Copyright © 2015 Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/

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p27CK-DD overexpression induces EMT in immortal mammary epithelial cells. (a and b) MCF-12A was transduced with the indicated lentiviral p27 vectors and effects on migration and matrigel invasion are represented relative to vector-only controls. (c) MCF-12A were transduced with control vector, C, p27CK− or p27CK-DD and morphology demonstrated by phase-contrast microscopy. (d–f) MCF-12A were transduced with control vector, C, or p27CK-DD and compared as follows: western blot for EMT markers and TWIST1 (d), QPCR for EMT transcription factors (e) and immunofluorescence for indicated proteins (f). (g) Effects of TWIST1 knockdown on EMT markers in MCF-12A-p27CK-DD cells. (h and i) Transwell migration (h) and matrigel invasion (i) of MCF-12A-C and MCF12A-p27CK-DD are shown with or without TWIST1 knockdown in MCF-12A-p27CK-DD. All data graphed represent mean of at least three repeats±s.e.m. *P<0.05 by Student's t-test (also refer to Supplementary Figures S1–S3).