Table 2.

Biochemical profile of C57Bl/6J mice fed either a C or a high fructose/high TFD for 8 wk and then challenged with an acute 4-h infusion of BCAA mixture

	C		TFD
	Saline	BCAA	Saline	BCAA
Body weight, g	25 ± 1	23 ± 1	28 ± 1*	28 ± 1*
Fasting glucose, mg/dl	112 ± 12	112 ± 5	120 ± 7	120 ± 10
Fasting plasma insulin, ng/ml	0.16 ± 0.01	0.16 ± 0.02	0.17 ± 0.01	0.17 ± 0.02
Fasting FFA, mM	0.41 ± 0.05	0.47 ± 0.03	0.47 ± 0.04	0.43 ± 0.03
Liver triglyceride, mg/g	44 ± 15	53 ± 10	159 ± 20*	160 ± 24*
Plasma BCAA, μM
Leucine	215 ± 24	378 ± 25#	168 ± 8	329 ± 24#
Isoleucine	153 ± 13	369 ± 19#	129 ± 6	346 ± 23#
Valine	229 ± 28	514 ± 28#	183 ± 7	449 ± 38#

Values are means ± SE (n = 6–7/group).

C, control diet; TFD, trans-fat diet.

^*P ≤ 0.05 between C and TFD;

^#P ≤ 0.05 between saline and BCAA.