Fig. 10.

Differential USF occupancy in the CPT1B promoter in HSkMC from lean and obese women. A: Western blot for USF-1, USF-2, and β-tubulin (loading control) of lysates from HSkMC pools. B: ChIP assay for USF-1/2 occupancy in the CPT1B promoter. Open bars, lean subjects; gray bars, obese subjects. Data are expressed as means ± SE obtained from triplicate cultures of HSkMC prepared from cells pooled from four lean and four obese subjects (Table 2), normalized to the lean control group. IgG, negative control ChIP. NC, negative control qPCR. Means not connected by a common letter are significantly different as determined by ANOVA and post hoc Tukey HSD multiple comparison test (P < 0.05).