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. 2015 Jun 24;309(4):C271–C281. doi: 10.1152/ajpcell.00366.2014

Fig. 1.

Fig. 1.

Vascular smooth muscle cell (vSMC) derivative organization and alignment after short-term cyclic uniaxial strain. A: schematic representation of human pluripotent stem cell (hPSC)-vSMC differentiation in vitro. B: smooth muscle-like cells (SMLCs; H9 cell line) at 5, 7, and 10% elongation. Light microscopy images, actin fiber labeled with phalloidin (green) (i) and quantification of cell orientation after 24-h uniaxial strain (ii). C: actin fiber labeled with phalloidin (green) of human embryonic stem cell (hESC) SMLCs embedded in collagen gel at 7% elongation. D: hPSC derivatives (H9 hESCs) and aortic vSMCs control cell line exposed to uniaxial strain at 7% elongation for 24 h. Light microscopy images (i), immunofluorescence images of phalloidin (green) and calponin (red; nuclei in blue) (ii), and quantification of cell orientation before (open) and after (solid) uniaxial strain (iii). Arrows indicate direction of strain. All graphical data are reported as means ± SE. ***P < 0.001. mSMLCs, mature SMLCs; Con-vSMCs, contractile vSMCs.