Fig. 3.
ECM gene expression of hPSC-vSMC derivatives after long-term cyclic uniaxial strain. A: schematic representation of long-term uniaxial strain experiments. Mech-mSMLCs, mechanically strained mSMLCs. B: SMLC and mSMLC collagen type I, collagen type III, collagen type IV, fibronectin, and elastin gene expression after 6 days of long-term uniaxial strain in serum-deprived media using quantitative real-time RT-PCR. C: immunofluorescence images of mSMLC elastin protein deposition after 24-h uniaxial strain: phalloidin (green), elastin (red), nuclei (blue). All graphical data are reported as means ± SE. *P < 0.05, **P < 0.01, and ***P < 0.001.