Fig. 4.

Experimental setup to study the effect of circumferential strain using a pulsatile flow bioreactor. A: schematic representation of the experimental setup of pulsatile flow bioreactor where media is propelled inside system tubing by a peristaltic pump and travels into a 30-gauge needle then into a silicone construct, where circumferential strain is generated. The medium then travels from the silicone construct and out of a secondary 30-gauge needle and is finally recycled in a media reservoir. PDMS, polydimethylsiloxane. B: schematic representation of long-term uniaxial strain experiments. C: confocal images of a cross section and 3D reconstruction of a flexible silicone tube seeded with hPSC mSMLCs before pulsatile flow: phalloidin (green), nuclei (blue).