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4-HIL reduced phospho-IRS-1 levels. Treatment with 4-HIL led to the reduced expression of phospho-IRS-1 (Ser307). HepG2 cells (2×106) were treated with TNF-α alone or TNF-α plus 20 µM4-HIL for 24 h. Whole cell extracts were prepared to assess the expression of phospho-IRS-1. The same blots were stripped and reprobed with β-actin antibody to show equal protein loading (***P<0.001 and *P<0.05 vs. TNF-α group).
