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. 2015 Jun 15;8(6):8626–8633.

Figure 4.

Figure 4

4-HIL reduced phospho-AKT levels. Treatment with 4-HIL led to the reduced expression of phospho-AKT (Ser473). HepG2 cells (2×106) were treated with TNF-α alone or TNF-α plus 20 µM4-HIL for 24 h. Whole cell extracts were prepared to assess the expression of phospho-AKT. The same blots were stripped and reprobed with β-actin antibody to verify equal protein loading (***P<0.001 and *P<0.05 vs. TNF-α group).