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Primers used for real-time RT-PCR
| Target gene | Primer sequences | Amplicon (bp) | |
|---|---|---|---|
| TNF-a | FP | 5’-ATGAGCACGGAAAGCATG-3’ | 276 |
| RP | 5’-TACGGGCTTGTCACTCGAGTT-3’ | ||
| IL-6 | FP | 5’-GACAAAGCCAGAGTCATTCA-3’ | 229 |
| RP | 5’-ACTAGGTTTGCCGAGTAGAC-3 | ||
| IL-1β | FP | 5’-TTGTGGCTGTGGAGAAGCTG-3’ | 377 |
| RP | 5’-GCCGTCTTTCATACACAGG-3’ | ||
| GAPDH | FP | 5’-AAC CTG CCA AGT ATG ATG-3’ | 119 |
| RP | 5’-GGA GTT GCT GTT GAA GTC-3’ |
