Fig. 6.

WNT/β-catenin signaling increases the expression of miR-124. Real-time PCR analysis of miR-124 levels in fAEC II (A) or E16 fetal lung slice (B) treated with 50% WNT3a conditioned medium or control L-cell conditioned medium (CON) for 24 h (A) or 5 days (B). Real-time PCR analysis of miR-124 in MLE15 cells with transient transfection of ΔGSK-β-catenin or control for 24 h (C). The expression level of miR-124 was calculated using ΔCt formula: 2^{−(CtmiRNA−Ct18S)} (A and C). Data was further expressed as a ratio to CON in B. Results are presented as means ± SD from 3 independent experiments. *P < 0.05 vs. CON or control (Student t-test).