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. 2015 Jun 12;309(4):L400–L413. doi: 10.1152/ajplung.00356.2014

Fig. 7.

Fig. 7.

Effect of ΔGSK β-catenin and LEF1 overexpression on miR-124 promoter activities in MLE15 cells. A: putative LEF1 binding sites in human miR-124 promoters identified by TRANSFAC software (version 8.3). miR-124-1 is transcribed from the minus strand of genomic DNA. B and C: MLE15 cells were cotransfected with human miR-124-1 (−1,753 to +36), miR-124-2 (−2,146 to +46), or miR-124-3 (−1,770 to +36) luciferase promoter construct (50 ng/well) and ΔGSK β-catenin expression plasmid (20 ng/well), LEF1 expression plasmid (20 ng/well) or empty vector. Dual luciferase activities were expressed as ratios of firefly to Renilla luciferase activities. Data shown are induction fold over respective control empty vector. Results are presented as means ± SD from 3 independent transfection experiments performed in triplicate. *P < 0.05 vs. empty vector, **P < 0.01 vs. empty vector (Student t-test).