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. 2015 Aug 17;6:806. doi: 10.3389/fmicb.2015.00806

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Copyright © 2015 Hu and Zhu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Shiga toxin production in E. coli O157:H7 EDL933 strains. (A) Relative mRNA expression; (B) Relative protein content. EDL933: wild-type E. coli O157:H7 strain; EDL933 Δpnp: EDL933 pnp deletion mutant strain; pBBR1: EDL933 Δpnp strain carrying an empty vector pBBR1; pBBR1::pnp: EDL933 Δpnp strain complemented with pBBR1::pnp. □: EDL933; ■: EDL933Δpnp; ***P < 0.001 (Mean ± SEM; n = 4).