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Effects of PNPase on the virulence factors of E. coli O157:H7 EDL933, Sakai, and 86-24 strains. (A) Stx 2A and Tir protein contents by immunoblotting; (B) stx2 phage DNA content by qPCR. □: wild-type (WT); ■: pnp deletion mutant (Δpnp). ***P < 0.001 (Mean ± SEM, n = 4).
