Table 1.
Primer sets used for quantitative Reverse Transcription PCR (qRT-PCR) for Shiga toxin (Stx) and phage related genes.
| Gene name | Product size | Direction | Sequence |
|---|---|---|---|
| cI | 80 bp | Forward | TCACACGAAGACCAAAGGCA |
| Reverse | TGCCATCGAGATGACCGAAG | ||
| cII | 118 bp | Forward | ACCTGTCAACGCTTACCCAG |
| Reverse | GATGCCATGCCAAAAGCACA | ||
| cIII | 101 bp | Forward | TTCTTTGGGACTCCTGGCTG |
| Reverse | GGCTGCCCTTCCGAATCTTT | ||
| cro | 75 bp | Forward | GAAGTTGCGAAGGCTTGTGG |
| Reverse | AGTCTTAGGGAGGAAGCCGT | ||
| gapA | 103 bp | Forward | CCAGGACATCGTTTCCAAC |
| Reverse | GGTGGTCATCAGACCTTCG | ||
| O | 104 bp | Forward | GATGCTGCAATTCAGAGCGG |
| Reverse | TTTCTGGCTGATGGTGCGAT | ||
| rcsA | 124 bp | Forward | CTCGACGATATCCTTGGCGA |
| Reverse | CCTGACCTGCCATCCACATT | ||
| recA | 70 bp | Forward | CTGGGAGCACAGCAGGTTGTCG |
| Reverse | TGAACACGCGCTGGACCCAATC | ||
| S | 124 bp | Forward | AGTTGCTGGACAGGGTTTCC |
| Reverse | GCCTTACGCCGGTCTTCTTT | ||
| stx1 | 180 bp | Forward | ATAAATCGCCATTCGTTGACTAC |
| Reverse | AGAACGCCCACTGAGATCATC | ||
| stx2A | 133 bp | Forward | CGTCACTCACTGGTTTCATCAT |
| Reverse | TCTGTATCTGCCTGAAGCGTAA |