Table 1.
Bacterial strains and plasmids used in this study.
| Strains or plasmids | Relevant characteristics | Source or reference |
|---|---|---|
| E. coli K12 ER2925 | dcm-6 dam13::Tn9 | New England Biolabs |
| B. amyloliquefaciens subsp. plantarum strain AP193 | Wild type | Dr. Joseph Kloepper (Department of Entomology and Plant Pathology, Auburn University) |
| AP193Δsfp | Deficient in lipopeptides and polyketides | This study |
| AP193ΔsrfAA | Deficient in surfactin production | This study |
| AP193ΔdfnD | Deficient in difficidin production | This study |
| Bacillus amyloliquefaciens FZB42 | Wild type | Chen et al., 2007 |
| pMK4 | E. coli-Bacillus shuttle plasmid, rolling circle replicative, CmR | BGSC |
| pNZT1 | Replication thermosensitive derivative of the rolling-circle plasmid pWV01 (pG+ replicon, EmR) | Xiaozhou Zhang, Virginia Tech |
| pNZ-sfp | pNZT1 with upstream and downstream sequences of gene sfp | This study |
| pNZ-srf | pNZT1 with knock-out construct of srfAA | This study |
| pNZ-dif | pNZT1 with knock-out construct of dfnD | This study |