Table 3.
Details of PCR-RFLP of the studied SNPs.
| SNP | Primer sequences | Annealing temp. (°C) | Product size (bp) | Enzyme | Genotype | Fragment sizes (bp) |
|---|---|---|---|---|---|---|
| rs1801320 | (F) 5′TGGGAACTGCAACTCATCTGG3′ (R) 5′GCGCTCCTCTCTCCAGCAG3′ |
GG | 71, 86 | |||
| 65 | 157 | MvaI | GC | 71, 86, 157 | ||
| CC | 157 | |||||
|
| ||||||
| rs3218536 | (F) 5′CGTCAATGGAGGAGAAAGTGTG3′ (R) 5′TCGAGAGGCATGAGAAGGTT3′ |
GG | 166 | |||
| 64 | 166 | SexA1 | GA | 79, 87, 166 | ||
| AA | 79, 87 | |||||
|
| ||||||
| rs861539 | (F) 5′TAAGAAGGTCCCCGTACTCC3′ (R) 5′CTGCGCATCAACCAGGTGAG3′ |
CC | 34, 161 | |||
| 64 | 195 | NlaIII | CT | 56, 105, 161 | ||
| TT | 56, 105 | |||||