Figure 2.
Influence of Cd on the cell viability of RAW 264.7 macrophages. Cell viability was determined using the WST-1 assay (Roche Diagnostics). 2 × 105 cells/well were seeded in a 96-well microculture plate and subsequently stimulated with various Cd concentrations alone or in the presence of hk S.E. After 24 and 48 h WST-1 Reagent was added for 1 h followed by determination of the optical density in supernatants at 437 nm using a conventional microplate reader (Saphire2, Tecan). Data represent the mean values and are representative of three independent experiments (n = 2 per experiment). ∗ indicates significant (p < 0.05) differences compared to control.