Figure 4.
Influence of Cd on chemokine and NO production of RAW 264.7 macrophages. A volume of 10 mL containing 1 × 106 cells/mL was seeded in a culture dish and subsequently stimulated with 0.1 μM or 10 μM Cd in the presence of hk S.E. After indicated periods of incubation supernatants were collected and chemokine (i.e., CXCL1 (a)) and nitrite/nitrate concentrations (b) were determined by ELISA or Griess reaction, respectively. Data represent the mean ± S.E.M. and are representative of three independent experiments (n = 3).