Table 1.
Cadmium atoms per cell in RAW 264.7 macrophages. Cells were incubated for 2 h, 4 h or 20 h with 0.01 µM, 0.1 µM or 10 µM CdCl2, or without CdCl2 for control, each in the presence or absence of hk S.E. Afterwards cells were harvested, washed, mineralized and the number of cadmium atoms per cell was determined by ICP-MS. Shown are the data derived from 2–6 independent biological replicates with standard deviation. (n.d. not determined)
0 µM CdCl2 | 0.01 µM CdCl2 | 0.1 µM CdCl2 | 10 µM CdCl2 | |||||
---|---|---|---|---|---|---|---|---|
−hk S.E. | +hk S.E. | −hk S.E. | +hk S.E. | −hk S.E. | +hk S.E. | −hk S.E. | +hk S.E. | |
2 h |
2.63 ×104
± 2 × 104 |
2.19 ×104
± 1 × 104 |
1.13 ×105
± 3 × 104 |
2.11 ×105
± 1 × 105 |
1.04 ×106
± 7 × 104 |
9.30 ×105
± 3 × 104 |
1.65 ×107
± 7 × 105 |
1.52 ×107
± 2 × 106 |
| ||||||||
4 h |
1.35 ×104
± 6 × 103 |
1.97 ×104
± 1 × 104 |
2.20 ×105
± 7 × 104 |
2.89 ×105
± 1 × 105 |
2.25 ×106
± 1 × 106 |
3.03 ×106
± 2 × 106 |
n.d. | n.d. |
| ||||||||
20 h |
4.02 ×103
± 9 × 103 |
3.21 ×104
± 4 × 104 |
n.d. | n.d. | n.d. | n.d. |
1.81 ×108
± 2 × 107 |
2.01 ×108
± 1 × 107 |