Each data point corresponds to one histoblast nest. The leading edge of each histoblast nest was tracked using imageJ. Speed was calculated measuring total distance covered (micrometer/hour). Genotypes: Control was
esg-GAL4, UAS-GFP.
esg-GAL4 and
UAS-GFP were recombined onto the KO1 and KO2 chromosomes and onto the
miR-965-Rescue chromosome. Data include examples with both recombinant mutant chromosomes. No difference between these two recombinants was apparent. n = 18 for the
miR-965 (KO1/KO2) mutant combination. n = 15 for control and rescue. Left panel: p < 0.0001 comparing KO1/KO2 with control, p < 0.01 comparing KO1/KO2 with rescue using one-way ANOVA. Right panel: p < 0.001 comparing KO1/KO2 with control, p < 0.01 comparing KO1/KO2 with rescue using one-way ANOVA. Refers to
Figure 3B and
Videos 5–7.