(A) Influence of the GABAA receptor antagonist PTX on the effect of exogenous application of GABA of VTA-DA neurons in rats. Left panel: typical current traces of sEPSC before and after GABA (10 μM) in the presence of PTX (100 μM). Middle panel: typical time course of the frequency of sEPSCs before and after GABA (10 μM) in the presence of PTX (100 μM). Right panel: average frequency of sEPSCs before and after GABA (10 μM) in the presence of PTX (100 μM) (n = 6 cells from four rats, p < 0.05, compared to PTX before GABA). (B) Influence of the GABAB receptor antagonist CGP54626 on the effect of exogenous application of GABA of VTA-DA neurons in rats. Left panel: typical current traces of sEPSC before and after GABA (10 μM) in the presence of CGP54626 (2 μM). Middle panel: typical time course of the frequency of sEPSCs before and after GABA (10 μM) in the presence of CGP54626 (2 μM). Right panel: average frequency of sEPSCs before and after GABA (10 μM) in the presence of CGP54626 (2 μM) (n = 6 cells from four rats, p = 0.87). (C) Influence of the GABAB receptor antagonist CGP54626 on 470 nm light-induced inhibition of the frequency of sEPSCs of VTA-DA neurons in mice. Left panel: typical current traces of sEPSCs before and after blue light (470 nm) stimulation in the presence of CGP54626 (2 μM). Middle panel: typical time course of the frequency of sEPSCs before and after blue light (470 nm) stimulation in the presence of CGP54626 (2 μM). Right panel: average frequency of sEPSCs before and after blue light (470 nm) stimulation in the presence of CGP54626 (2 μM) (n = 6 cells from five mice, p = 0.21). Data are shown as the mean ±s.e.m. *p < 0.05.
DOI:
http://dx.doi.org/10.7554/eLife.09275.008