Table 1.
The inhibition coefficients I 24 (%) for 24 h exposure and I 48 (%) for 48 h exposure of benzonitrile pesticides and their microbial metabolic products on Hep G2 cells for concentrations 25 mg/L, 50 mg/L, and 100 mg/L.
| Compounds |
I
24 (%) 25 mg/L |
I
48 (%) 25 mg/L |
I
24 (%) 50 mg/L |
I
48 (%) 50 mg/L |
I
24 (%) 100 mg/L |
I
48 (%) 100 mg/L |
|---|---|---|---|---|---|---|
| BRX | 20 ± 1 | 45 ± 3 | 52 ± 1 | 77 ± 2 | 92 ± 4 | 95 ± 3 |
| BRAC | 8 ± 0,4 | 11 ± 1 | 10 ± 1 | 10 ± 1 | 15 ± 1 | 20 ± 2 |
| BRAM | ND | ND | ND | 9 ± 0,5 | ND | 12 ± 2 |
| CHX | 50 ± 3 | 72 ± 2 | 70 ± 4 | 90 ± 2 | 90 ± 2 | 99 ± 1 |
| CHXAC | 10 ± 1 | 17 ± 2 | 25 ± 3 | 35 ± 2 | 50 ± 2 | 56 ± 3 |
| CHXAM | ND | 9 ± 1 | ND | 4 ± 0,1 | 10 ± 1 | 13 ± 1 |
| DCB | ND | ND | ND∗ | 14 ± 1∗ | ND∗ | 15 ± 2∗ |
| DCBA | ND | ND | ND | ND | 20 ± 2 | 12 ± 1 |
| BAM | ND | 3 ± 0,2 | ND | 4 ± 0,2 | 10 ± 1 | 13 ± 3 |
| IOX | 50 ± 3 | 58 ± 3 | 70 ± 4 | 88 ± 5 | 100 ± 5 | 100 ± 4 |
| IOXAC | ND | 3 ± 0,2 | ND | 10 ± 2 | ND | 19 ± 2 |
| IOXAM | ND | ND | ND | ND | ND | 16 ± 1 |
BRX: 3,5-dibromo-4-hydroxybenzonitrile, BRAC: 3,5-dibromo-4-hydroxybenzoic acid, BRAM: 3,5-dibromo-4-hydroxybenzamide, DCB: 2,6-dichlorobenzonitrile, DCBA: 2,6-dichlorobenzoic acid, BAM: 2,6-dichlorobenzamide, CHX: 3,5-dichloro-4-hydroxybenzonitrile, CHXAC: 3,5-dichloro-4-hydroxybenzoic acid, CHXAM: 3,5-dichloro-4-hydroxybenzamide, IOX: 3,5-diiodo-4-hydroxybenzonitrile, IOXAC: 3,5-diiodo-4-hydroxybenzoic acid, and IOXAM: 3,5-diiodo-4-hydroxybenzamide; ND: below detection limit; ∗: the solubility of DCB in water is around 20 mg/L; therefore the inhibitions at concentrations 50 and 100 mg/L are only indicative; ±: standard deviation of 3 parallels.