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. 2015 Aug 17;6:128. doi: 10.3389/fendo.2015.00128

Figure 4.

Figure 4

Activation of guinea pig and guinea pig–human chimeric receptors by RLN and ML290. (A) Porcine RLN-induced cAMP response. (B) ML290-induced cAMP response. HEK293T cells were transiently transfected with RXFP1 expression vectors and cAMP was determined using HTRF cAMP assay. cAMP activity is expressed as the percentage of 10 μM Forskolin-stimulated response. Data are expressed as mean ± SEM, each point represent triplicate measurements. The experiment was repeated three times. *p < 0.05, **p < 0.01, ***p < 0.001 compared to hRXFP1.