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. 2015 Aug 17;6:128. doi: 10.3389/fendo.2015.00128

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Copyright © 2015 Huang, Myhr, Bathgate, Ho, Bueno, Hu, Xiao, Southall, Barnaeva, Agoulnik, Marugan, Ferrer and Agoulnik.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Activation of rabbit and rabbit–human chimeric receptors by RLN and ML290. (A) Porcine RLN-induced cAMP response. (B) ML290-induced cAMP response. HEK293T cells were transiently transfected with RXFP1 expression vectors and cAMP was determined using the HTRF cAMP assay. cAMP activity is expressed as the percentage of hRXFP1 cAMP activation by 10 nM of RLN. Data are expressed as mean ± SEM; each point represents triplicate measurements. The experiment was repeated at least three times. ***p < 0.001 compared to hRXFP1.