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. 2015 Aug 17;6:128. doi: 10.3389/fendo.2015.00128

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Copyright © 2015 Huang, Myhr, Bathgate, Ho, Bueno, Hu, Xiao, Southall, Barnaeva, Agoulnik, Marugan, Ferrer and Agoulnik.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Rabbit RXFP1 does not bind relaxin peptide. (A) Total and cell surface expression of hRXFP1 and R1-RXFP1. Expression is normalized to the expression of human receptor. ***p < 0.001 compared to hRXFP1. (B) Saturation binding using Eu-labeled H2 RLN. (C) Human RLN-induced cAMP response. (D) ML290-induced cAMP response. cAMP activity is expressed as the percentage of the 5 μM Forskolin-stimulated response for each receptor. Data are expressed as mean ± SEM; each point represents triplicate measurements. The experiment was repeated at least three times.