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. 2015 Aug 17;5:13142. doi: 10.1038/srep13142

Figure 2. Inhibiting WNT signaling with DKK1 promoted osteogenic differentiation of D-PDLSCs.

Figure 2

(A) To examine whether NF-κB signaling mediated impaired osteogenic potential of D-PDLSCs, we treated D-PDLSCs with NF-κB inhibitor PDTC. H-PDLSCs, P-PDLSCs and P-PDLSCs with PDTC were as control. The activation of NF-κB (phosphorylated p65, pNF-κB), RUNX2 and ACTIN was examined by western blot analysis. (B) H-PDLSCs, P-PDLSCs and D-PDLSCs were induced 7 days by osteogenic medium. The expression of DKK1 was examined by Real-time PCR (n = 4). (C) Quantification of Alizarin Red staining. PDLSCs for staining were induced to osteogenic differentiation for 28 days (n = 5), bar = 200 μm. (D,E) Real-time PCR and western blot analysis of the osteoblast marker gene (RUNX2, normalized to β-actin) on day 7 (n = 3). Active β-catenin which indicates WNT signaling is activated was examined by western blot analysis. Data (±SD) are representative of two (D) or three (B,C) independent experiments. Student’s t test was performed to determine statistical significance (*p < 0.05, **p < 0.01).