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. 2015 Aug 17;5:13142. doi: 10.1038/srep13142

Figure 4. Inhibition of WNT could rescue the osteogenic differentiation of D-PDLSCs and PDLSCs with AGEs.

Figure 4

(A) Western blot analysis of active β-Catenin in PDLSCs with AGEs were examined after DKK1 treatment. (B) The effect of β-Catenin siRNA was checked by western blot in H-PDLSCs and D-PDLSCs. (C,D) ALP activity was examined in H-PDLSCs and D-PDLSCs after treatment with siRNA for β-Catenin (n = 4). (E,F) Real-time RT-PCR and western blot analysis of the RUNX2 and RAGE on day 7 (n = 3). Data (±SD) are representative of three (C,D) independent experiments. Student’s t test was performed to determine statistical significance (*p < 0.05, **p < 0.01).