Table 5.
Experimental design and results of Box-Behnken optimization experiment.
| Trials | X 1 | X 2 | X 3 | Yield (mg/L) | |
|---|---|---|---|---|---|
| Observed | Predicted | ||||
| 1 | 22.00 | 24.00 | 36.00 | 73.66 ± 1.62 | 73.43 |
| 2 | 34.00 | 28.00 | 32.00 | 86.35 ± 2.78 | 86.78 |
| 3 | 28.00 | 28.00 | 28.00 | 77.37 ± 1.83 | 77.57 |
| 4 | 28.00 | 24.00 | 32.00 | 124.39 ± 1.92 | 124.15 |
| 5 | 22.00 | 28.00 | 32.00 | 71.97 ± 1.26 | 71.81 |
| 6 | 34.00 | 24.00 | 28.00 | 94.52 ± 3.73 | 94.75 |
| 7 | 28.00 | 28.00 | 36.00 | 92.97 ± 5.06 | 93.37 |
| 8 | 28.00 | 20.00 | 28.00 | 115.92 ± 2.90 | 115.53 |
| 9 | 22.00 | 20.00 | 32.00 | 86.35 ± 2.72 | 85.93 |
| 10 | 28.00 | 24.00 | 32.00 | 124.22 ± 1.24 | 124.15 |
| 11 | 28.00 | 24.00 | 32.00 | 123.85 ± 1.63 | 124.15 |
| 12 | 34.00 | 20.00 | 32.00 | 92.67 ± 3.56 | 93.37 |
| 13 | 34.00 | 24.00 | 36.00 | 90.80 ± 2.90 | 90.82 |
| 14 | 22.00 | 24.00 | 28.00 | 91.98 ± 3.72 | 91.95 |
| 15 | 28.00 | 20.00 | 36.00 | 77.48 ± 2.35 | 77.28 |
Each experiment was repeated three times, and all of the data were expressed as means ± standard deviations. X 1, X 2, and X 3 represent corn powder (g/L), soybean meal, and incubation temperature (°C), respectively.