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ATP-based luminescent proliferation assays with Atf4 wildtype (wt) and knockout (−/−) (A) and Chop wt and −/– (B) MEF at 16 h. qRT-PCR for UPR and apoptotic transcripts in Atf4 wt and −/– (C) or eIF2α wt (S/S) and Ser51Ala mutant (A/A) (D) in MEF 40 μM lobophorin F 4 or 2.5 μg/mL Tm 6 h.
