TABLE 2.
Mutations detected in mutants M1 to M9
| Mutant | EDAGa | Mutation | Predicted impact of the mutation |
|---|---|---|---|
| M1 | 00077 | C302G | T101R substitution in Ser Thr protein phosphatase StpA |
| 01693 | G5A | S2N substitution in ribosome maturation factor RimP | |
| 00369 | C623T | A208V substitution in signal recognition particle-docking protein FtsY | |
| 00670 | G979T | D327Y substitution in mannitol operon transcriptional antiterminator | |
| 01067 | C34T | R12C substitution in glyceraldehyde-3-phosphate dehydrogenase, type I | |
| 01287 | C486T | Silent mutation (member of the xanthine/uracil permease family) | |
| A61T | Intergenic, 61 bp upstream from EDAG_00488 | ||
| M2 | 02370 | C404A | A135E substitution in response regulator CroR |
| 00077 | C104T | A35V substitution in serine/threonine protein phosphatase StpA | |
| 00473 | C1616A | T539K substitution in PBPB | |
| 00961 | ΔA1726 | Truncation of DNA mismatch repair protein MutL | |
| 01903 | ΔA13 | Truncation of a putative NADPH-dependent FMN reductase | |
| ΔA360 | Intergenic, 360 bp downstream from EDAG_01362 | ||
| M3 | 00792 | A956G | Q319R substitution in a hypothetical protein |
| 02385 | A253G | T85A substitution in a hypothetical protein | |
| 01714 | A316G | N106D substitution in substrate-binding protein of a putative multiple sugar ABC transporter | |
| 00246 | iA158 | Truncation of a transcriptional regulator of the MarR family | |
| 00858 | ΔA19 | Truncation of glutamine-binding protein GlnH (ABC transporter) | |
| M4 | 00953 | C47T | P16L substitution in a putative phosphotyrosine protein phosphatase |
| 00202 | T542C | V181A substitution in 2-C-methyl-d-erythritol 4-phosphate cytidylyltransferase | |
| 01377 | G559A | D187N substitution in a putative DNA-directed DNA polymerase | |
| 00066 | iA511 | Truncation of a hypothetical protein | |
| 01651 | ΔA837 | Truncation of copper-translocating P-type ATPase | |
| 01550 | G165A | M55I substitution in response regulator DdcR | |
| M512 | 01551 | A481G | T161A substitution in sensor kinase DdcS |
| 01065 | T102C | Silent mutation in RNA polymerase sigma factor 54 gene | |
| 01143 | C97T | R33C substitution in ribosomal protein S21 | |
| 01730 | C98T | A33V substitution in a putative phage DNA packaging protein | |
| 00694 | C1498T | Truncation of a putative calcium-transporting ATPase | |
| 02113 | A1377G | Silent mutation (large subunit of a putative phage terminase) | |
| 01186 | A547G | S183G substitution in a putative sensor kinase | |
| 00615 | A778G | T260A substitution in RNase HIII | |
| 00746 | A549G | Silent mutation (putative sensor kinase) | |
| 01598 | C74A | P25H substitution in a phosphoesterase belonging to the DHH family | |
| 00511 | A530G | D177G substitution in glucose-1-phosphate thymidylyltransferase | |
| 00961 | ΔA661 | Truncation of DNA mismatch repair protein MutL | |
| 00469 | iG147 | Truncation of a putative lipoprotein protein | |
| 00089 | ΔA24 | Truncation of l-lactate dehydrogenase | |
| 00626 | ΔA1994 | Truncation of a putative V-type ATPase | |
| M6 | 00718 | ΔA178 | Truncation of a hypothetical protein |
| 01553 | G379A | E127K substitution in the catalytic domain of DdcY | |
| 00728 | C1556T | A519V substitution in ATP-dependent DNA helicase IV | |
| 01753 | T623C | L208S substitution in a hypothetical protein | |
| 01577 | C2248T | P750S substitution in valyl-tRNA synthetase | |
| 02299 | A29G | H10R substitution in a hypothetical protein | |
| 02038 | T159C | Silent mutation (hypothetical ATP/GTP-binding protein) | |
| 01180 | ΔA463 | Truncation of a putative member of the YitT family | |
| 00623 | ΔA518 | Truncation of fructose-1,6-bisphosphatase | |
| 02047 | ΔA1033 | Truncation of a hypothetical PTS system transcriptional activator | |
| 01858 | ΔT20 | Truncation of a hypothetical protein | |
| M7 | 02391 | C70T | Truncation of a putative sodium/dicarboxylate symporter |
| 00183 | C482T | P161L substitution in a hypothetical protein | |
| 01207 | A517G | T173A substitution in a hypothetical protein | |
| 01042 | G901A | A301T substitution in a sensor histidine kinase | |
| 01754 | T86C | V29A substitution in membrane-bound protein LytR | |
| 00781 | ΔA482 | Truncation of a hypothetical protein | |
| 01454 | ΔA234 | Truncation of a member of a Spx/MgsR transcriptional regulator family | |
| ΔA32 | Intergenic, 32 bp downstream from EDAG_01142 | ||
| ΔT101 | Intergenic, 101 bp downstream from EDAG_05560 | ||
| ΔA147 | Intergenic, 147 bp downstream from EDAG_00488 | ||
| M8 | 00248 | G139C | D47H substitution in acyl carrier protein |
| 00719 | G1461A | Truncation of glycerol kinase | |
| 02004 | ΔA114 | Truncation of ferrous iron transporter B | |
| 02466 | ΔA9 | Truncation of d,d-carboxypeptidase DdcP | |
| M9 | 01136 | G169A | G57R substitution in a putative alpha-galactosidase |
| 02125 | A278T | H93L substitution in a hypothetical protein | |
| 00476 | G592A | A198T substitution in transferase MurG | |
| 00308 | C342T | Silent mutation in permease OpuCB gene | |
| 00549 | C419T | T140I substitution in DNA topoisomerase III | |
| 02412 | T476A | I159N substitution in phosphate-binding protein PstS | |
| 02124 | ΔT1893 | Truncation of phage tail tape measure protein | |
| 01553 | T41A | I14N substitution in the membrane anchor of DdcY | |
| 00606 | ΔA297 | Truncation of a hypothetical protein | |
| 01532 | ΔA117 | Truncation of a hypothetical RNA-binding | |
| 01554 | C614T | A205V substitution in MurAB | |
| 01561 | ΔA290 | Truncation of beta-glucoside specific transporter | |
| 00718 | A414T | Silent mutation (conserved hypothetical protein) | |
| C122T | Intergenic, 122 bp downstream from EDAG_02398 | ||
| ΔT262 | Intergenic, 262 bp downstream from EDAG_00339 |
a
EDAG number as appearing in the D344SRF genome database at http://www.broadinstitute.org/annotation/genome/enterococcus_rice/MultiHome.html.