FIG 2.

Time course of the activity of salinomycin, monensin, or nigericin on mature gametocytes. Gametocytes were incubated with the ionophores at 1 nM (circles), 10 nM (triangles), or 100 nM (squares) for different lengths of time (2, 6, 24, 48, and 72 h). (a) The pLDH viability assay was performed at the end of each incubation time. (b) The compounds were removed after each incubation time, fresh medium was added, and the pLDH assay was performed at 72 h. The results are the means ± SD from two independent experiments in triplicate.