Alignment of the 3Cpro amino acid sequences of 15 RV (RV-A and -B) genotypes, EV68 (EV-D), and PV1 (EV-C). A yellow background indicates residues of the catalytic triad, a purple background indicates a residue that is mutated in both the SG85- and rupintrivir-resistant RV14 variants, a blue background indicates residues that are mutated in the SG85-resistant RV14 variant, a red background indicates residues that are mutated only in rupintrivir-resistant RV14 variants, and a green background indicates residues in the structure of the RV02 3Cpro that interact with compounds I and III (22) or residues in EV68 3Cpro that interact with SG85 (18). This sequence alignment was created in CLC sequence viewer 7 (Qiagen).