FIG 1.

Primer extension analysis of reverse transcriptase stops on 23S rRNA from E. coli JW2501-1 harboring the plasmid expressing the ClcD protein. The region shown is limited to the nucleotides flanking A2503. Lanes 1 to 4 (C, U, A, and G), dideoxy sequencing reactions. Lanes 5 to 8, primer extension reactions on total RNA from cells harboring the indicated plasmids. Reverse transcriptase stops 1 nucleotide before the corresponding nucleotide in the sequencing lanes. Arrow, stop mediated by modification from Cfr or Cfr-like proteins.