TABLE 1.
Primers used for amplification, reverse transcription, and sequencing
| Locus | Primer name | Sequence (5′→3′)a | Positionb |
|---|---|---|---|
| aac(3) | EcoRI AacF | GGAATTCCGTTAAGGAGGCGGCATATGACTACAACCAAC | 988–1020 |
| BamHI AacR | GGGATCCGGCCTAAAGCTCCCGGATGTAGAGCAGCATG | 1458–1434 | |
| aac(3) | NdeI AacF | GGGCATATGACTACAACCAACGAGGATC | 1003–1026 |
| XhoI AacR | CCCTCGAGCTACTAAAGCTCCCGGATGTAGAG | 1452–1432 | |
| aac(3) | AacF | ATGACTACAACCAACGAGATC | 1006–1026 |
| aac(3) | AacR | CTAAAGCTCCCGGATGTAGAG | 1452–1432 |
| aac(3) | Aac intR | ACGTAGGCGAAACCAATATCGCC | 1193–1171 |
| tnpCx | TnpF | ATGTCATCGTCGATACCATCTGC | 387–409 |
| tnpCx | TnpR | AAGCTTTGGCATCGGCGTGCGTT | 865–843 |
| Kinase | KinR | GTTGCAACCTGGACCGAACCATTC | 1616–1593 |
a
Restriction sites are in bold; the ribosome-binding site is underlined; start and stop codons are in italics.
b
The coordinates refer to the 1,800-bp sequence presented in Fig. 2.