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Steady-state kinetic parameters for the AAC(3)-XI-catalyzed reaction
| Substrate | kcat (s−1) | Km (μM) | kcat/Km (M−1 s−1) |
|---|---|---|---|
| Acetyl-CoAa | 0.24 ± 0.01 | 2.7 ± 0.2 | (8.9 ± 0.8) × 104 |
| Dibekacin | 0.17 ± 0.01 | 9.2 ± 0.7 | (1.8 ± 0.2) × 104 |
| Kanamycin B | 0.23 ± 0.01 | 13.8 ± 1.2 | (1.7 ± 0.2) × 104 |
Kinetic parameters for the turnover of acetyl-CoA were determined using a fixed concentration of dibekacin, as described in Materials and Methods.
