FIG 2.

CANBEF-24 causes morphological changes characteristic of damage to the cell wall of A. fumigatus. A. fumigatus strain Af293 was incubated for 24 h in the presence of 0.8 μM CANBEF-24 and was analyzed microscopically, in comparison to untreated cells, by calcofluor white (CFW) staining and TEM (A) and DiBAC vital staining (B). Increased CFW staining of cell wall polysaccharides and abnormal cell wall morphology (TEM) reveal CANBEF-24-induced wall damage. DiBAC staining indicates partial cell death in discrete areas of the hypha.