TABLE 1.
Relative CYP450 enzyme activities of HLMsa
| HLM type | Relative CYP450 content | Ratio of CYP450 enzyme metabolic activity |
|||
|---|---|---|---|---|---|
| 2C19 | 2C9 | 3A4 | 3A4/5 | ||
| Pooled | |||||
| PMG1 | 1.6 | 3.3 | 1.2 | NA | 3.6 |
| PMG2 | 2.1 | 4.4 | 2.5 | 34.6 | 8 |
| Individual | |||||
| 2C19-WT | 1 | 1 | 1.9 | 1 | 1 |
| 2C9-WT | 1.4 | 1 | 1 | 6.1 | 3.9 |
a
Relative CYP450 content was calculated as the ratio of the total CYP concentration (in nanomoles per milligram of total protein) to the concentration of the HLM type with the lowest concentration, 2C19-WT. The metabolic activity ratios were calculated using the apparent activity of that enzyme in each HLM type on the basis of metabolism of a marker substrate (in picomoles per milligram per minute). All lot-specific information is from data sheets kindly provided by XenoTech, LLC. NA, not available.