FIG 2.

Blocking of LPS binding to TLR4 by Rg1 and Re. (A) LPS binding to macrophages pretreated with Rg1. RAW264.7 cells were pretreated with Rg1 for 1 h and then stimulated by Alexa Fluor 594-conjugated LPS (red). TLR4 was labeled with a specific antibody and a secondary antibody conjugated with DyLight 488 (green). Representative images from three independent experiments are shown. Bar, 10 μm. (B) LPS binding to macrophages pretreated with Re. RAW264.7 cells were treated in the same way as described for panel A except that Re was used in the pretreatment.