FIG 4.

Anti-inflammatory effect of Rg1 and Re in rats. (A and B) Effect of Rg1 and Re on body temperature and white blood cell counts in rats injected with LPS. Groups of rats (n = 6 per group) were intravenously injected with saline solution, Rg1, Re, or TAK-242 (1 mg/kg) 15 min before challenge with LPS (2.5 μg/kg). An additional group of rats were injected with saline solution only as a control. Body temperature was measured (A) and WBCs were counted (B) at the indicated time points. (C to E) Effect of Rg1 and Re on inflammatory mediators. Rats were treated in the same way as described for panel A. Blood samples were collected 4 h after LPS challenge for determination of TNF-α, IL-1β, IL-6, COX-2, and iNOS levels by Western blot analysis (C), and analyses of NO (D) and PGE2 (E) levels were performed using NO and prostaglandin E2 assay kits. Values are means ± SD (n = 6).*, P < 0.05; ** P < 0.01; ***, P < 0.001 (two-tailed Student's t test).