FIG 6.

Effect of Rg1 and Re treatment on WBC populations in mice. BALB/c mice were randomly assigned to 5 groups (9 mice per group). The mice were subcutaneously injected with 10 mg of Rg1/kg body weight (mg/kg Rg1), 20 mg/kg Re, or 5 mg/kg TAK-242 3 times at 30-min intervals or left untreated and were then challenged with 20 mg/kg LPS 15 min later. An additional group of mice were injected with LPS first and then subcutaneously administered 10 mg/kg Rg1 3 times. Three mice in each group were euthanized for blood collection at the indicated time points. Another 5 naive mice were euthanized and sampled at h 0. Total and differential WBC counts in the EDTA-treated blood samples were determined. Values are means ± SD. *, P < 0.05; **, P < 0.01; ***, P < 0.001 (compared to the group administered LPS alone by the two-tailed Student's t test).