FIG 7.

Antisepsis effect of Rg1 and Re on the release of proinflammatory mediators in mice. BALB/c mice were randomly assigned to 5 groups (9 mice per group). The mice were subcutaneously injected with 10 mg/kg Rg1, 20 mg/kg Re, or 5 mg/kg TAK-242 3 times at 30-min intervals or left untreated and then challenged with 20 mg/kg LPS 15 min later. An additional group of mice were injected with LPS first and then subcutaneously administered 10 mg/kg Rg1 3 times. Three mice in each group were euthanized for blood collection at the indicated time points. Another 5 naive mice were euthanized and sampled at h 0. Sera were prepared and used for the measurement of levels of proinflammatory cytokines and chemokines (A), PGE2 (B), and NO (C). Data are presented as means ± SD (A and B) or means (C). *, P < 0.05; **, P < 0.01; ***, P < 0.001 (compared to LPS alone group by the two-tailed Student's t test).