TABLE 1.

Gene-specific primer pairs

Process and gene	Primer name	Nucleotide sequence (5′ to 3′)
Complementation
ads-4	ads4-F	TGGTTACGTGTTCTGCGTCAGTATC
	ads4-R	TCAGTCCCTATAACGAACACCTCAC
N. crassa transformant and double-mutant screening
ads-4	ads4v-F	CTTTCCAACCCAACCATC
	ads4v-R	GTCCGCTATACTGCTGTCC
Construction of ads-4 overexpression strain
cfp	cfp-F	CGACCTCAAACCTCAACAAAC
	cfp-R	ATATCAGATCCGATGCTCTCTCTTTAGGGTGAG
ads-4	hismycads4-F	GAGAGAGCATCGGATCTGATATCATCGATTTAAAGC
	hismycads4-R	TTTGCCCTCGCGAGCACTAACGTGGAAAATC
trpc	trpc-F	TTAGTGCTCGCGAGGGCAAAGGAATAGAGTAG
	trpc-R	AAGCAGCCCAGTAGTAGGTTGA
Construction of Afu1g16460-knockout strain
Afu1g16460 upstream	Afu1g16460U-XhoI-F	CCGCTCGAGGCGGCCGCGATGTCCGAAAAAAGGCAGAGG
	Afu1g16460U-ClaI-SmaI-R	CCATCGATCCCGGGGATGTGCGTATGCACGAGGTTC
Afu1g16460 downstream	Afu1g16460D-ClaI-F	CCATCGATCCCCCCTCTTTCCTTTGTTCATG
	Afu1g16460D-BamHI-R	CGGGATCCCGTAGGTTTTCCCTCGTCTGAA
A. fumigatus transformant screening
Afu1g16460	Afu1g16460V-F	GGCTTCATTGGTCCGTGC
	Afu1g16460V-R	AAGCCATTCTCGCAAGCC
qRT-PCR analysis
ads-4	Qncu08744-F	TCGAACTCTTGGGACTGCCAGAAA
	Qncu08744-R	AAGGCATTCCGATTGAGTCCGCTA
Ncmmt2	Qncu07879-F	ACACCGTTTCTGCCCTCT
	Qncu07879-R	CCGCCAGCTCTATATCCA
erg5	Qncu05278-F	TTTCACCTTCCTCTTCGCTTCCCA
	Qncu05278-R	TCATCGACTCAAGCTGCTCCATGT
cdr4	Qncu05591-F	ACGCTTTGGAAATGGATGGTGACG
	Qncu05591-R	ATGAACAAGGCGACGGAAATGCAG
Ncrta2	Qncu05209-F	TGAGCAAGATCATTGTCCTAAT
	Qncu05209-R	AAATACCACAGCCATCTCAC
NcabcB	Qncu03776-F	CGGTGATGCAGGAAGTTATC
	Qncu03776-R	CTTCAACACCGCCACTAAA
Ncmnn4	Qncu03213-F	GGTGGTGGAACAAGCAGAT
	Qncu03213-R	GGTCTCCGTTGGAGAAGTTAG