TABLE 2.
Inhibition rates with azoles for Neurospora crassa WT, Δads-4, Δads-4::ads-4, and ads-4OE strains
Drug | Inhibition rate (%)a |
|||
---|---|---|---|---|
WT | Δads-4 | Δads-4::ads-4 | ads-4OE | |
KTC | 72.29 ± 0.02 | 90.33 ± 0.02b | 72.92 ± 0.02 | 67.93 ± 0.02c |
FLC | 61.25 ± 0.01 | 69.96 ± 0.01b | 62.63 ± 0.01 | 58.51 ± 0.01c |
Tested strains are the wild-type (WT) strain, the ads-4-knockout strain (Δads-4), the ads-4-complemented strain (Δads-4::ads-4), and the ads-4 overexpression strain (ads-4OE). Radii of colonies were measured after 24 h of inoculation for nontreated plates and 48 h for drug-treated plates, and growth rates of strains were calculated by the equation colony radius (mm)/incubation time (h). The means of the relative inhibition rates for each fungicide were calculated with the following equation: (growth rate on plates without fungicide − growth rate on plates with fungicide)/growth rate on plates without fungicide. Differences between the mutants and the WT strain were statistically analyzed with the Waller-Duncan test.
Significantly different from the WT strain, P < 0.01.
Significantly different from the WT strain, P < 0.05.