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. 2015 Aug 17;5:13120. doi: 10.1038/srep13120

Figure 2. The WNT5A haplotype with −5244 CC exhibited lower transcriptional activity.

Figure 2

(A) In individuals carrying different rs504849 genotypes, the mRNA level of WNT5A in the G/G genotype significant lower compared with that in the A/A genotype (P < 0.03). The actual values for each genotype group were as follows: A/A, 1.63 ± 0.80; A/G, 1.16 ± 0.77; G/G, 0.33 ± 0.25. All values have been normalized to the levels of GAPDH and represent the mean ± SD from 3 independent experiments. (B) Transcriptional levels of enhancer region (−4972 A/C + −5244GCCA/CC) and promoter region (−1926 A/G + 230 A/C) were respectively examined with luciferase assay in HEK293 cells. For the −4972 A/C + −5244GCCA/CC haplotypes, the luciferase expression level was significantly lower in the minor haplotype (CC-C haplotype) compared to that with the major haplotype (GCCA-A haplotype) (20% decreases). However, there were no significance differences in luciferase expression between major (A-A) and minor (G-C) haplotypes for −1926 A/G + 230 A/C haplotypes.