Fig. S2.
Related to Fig. 3. (A) Comparison of IC50 values of lipid synthesis inhibitors cerulenin and orlistat between representative glycolytic and lipogenic cell lines in short-term (3 d) viability assays. The mean and SD between cell lines belonging to the glycolytic vs. lipogenic subtype is plotted where each cell line is shown as one dot, representing the mean of three replicates. Asterisks denote a statistically significant difference by Mann–Whitney test (*P < 0.05, **P < 0.01, ***P < 0.001). Data from Dataset S7. (B) Comparison in baseline fatty acid (FA) uptake between representative glycolytic and lipogenic cell lines. Asterisks denote a statistically significant difference by t test (*P < 0.05, **P < 0.01, ***P < 0.001). (C) Comparison in percent growth in 3.75% delipidated serum:1.25% FBS (relative to 5% FBS) between representative glycolytic and lipogenic cell lines. Asterisks denote a statistically significant difference by Mann–Whitney test (*P < 0.05, **P < 0.01, ***P < 0.001). (D) Western blots showing 98% in vivo knockdown of LDHA levels in HPAC xenografts administered with doxycycline (1 mg/mL) for 8 d vs. 5% sucrose. (E) In vivo knockdown of LDHA results in 9% TGI in the HPAC shLDHA model of a lipogenic subtype tumor.